lasmonic sensing through bioconjugation of Ag nanoparticles: Towards the development of immunoassays for ultralow quantification of antigens in colloidal dispersions

EDUARDO A. CORONADO

Advanced Materials Letters

VBRI Press

Año: 2018 vol. 9 p. 456 - 456

0976-3961

he combination of the optical properties of silver nanoparticles with some of the tool-kits of the widespread used ELISAmethod for antigen quantification immunoassays, gives rise to an enzyme-free, low-cost, fast and more sensitive optical method denoted as Intensity Depletion Immuno-linked Assay (IDILA), that can be performed directly in colloidal dispersion without any immobilization of the capture antibody, antigen, secondary and primary antibodies on a substrate. The capabilities of the method for quantifying antigens at ultralow concentrations in colloidal dispersions as well as its performance are demonstrated for specific antigens of importance in medicine and in food science.